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. 2021 Jun 10;83(8):1182–1190. doi: 10.1292/jvms.21-0256

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©2021 The Japanese Society of Veterinary Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 3. — Multiplication of transposon mutants in macrophage J774.1. The number of macrophages with more than 10 bacteria was indicated in 200 macrophages at 2 and 24 hr post-infection. 1, wild-type; 2, putative DEAD/DEAH box helicase (REQ_05430) mutant; 3, oxidoreductase (REQ_10430) mutant; 4, acyl-CoA thioesterase II (REQ_25310) mutant; 5, hypothetical protein (REQ_26170) mutant; 6, putative HNH endonuclease (REQ_27330)/ putative Fis family transcriptional regulator (REQ_27340) mutant; 7, putative carotenoid oxygenase (REQ_28270) mutant; 8, putative nitrite reductase large subunit NirB1 (REQ_32930) mutant. Data represent the means and standard deviations from three independent experiments and are analyzed by the two-tailed unpaired Student’s t test (*P<0.01 vs. wild-type).