Table 1. Identification of the genes with transposons in the survival-defective mutants of Rhodococcus equi.

Class	Mutant	Location	Known or putative function	VapA expression
Amino acid transportand metabolism	M95-15	REQ_29700	Gamma-glutamyl kinase-GP-reductase multienzyme complex ProA	Normal
Nucleotide biosynthesisand metabolism	M79-5	REQ_39910	Orotate phosphoribosyltransferase PyrE	Normal
Fatty acid and phospholipid metabolism	M77-6	REQ_25310	Acyl-CoA thioesterase II	Normal
Biosynthesis of cofactors, prosthetic groups, and carriers	M73-2	REQ_17830	Putative pyrazinamidase	Normal
Transcription, RNA processing and degradation	M121-1	REQ_05430	Putative DEAD/DEAH box helicase	Normal
DNA replication, recombination, modification, and repair	M134-15	REQ_00040	DNA replication and repair protein RecF	Normal
	M178-25	REQ_19710	Putative Rec X regulatory protein	Normal
Transcriptional regulator	M165-8	REQ_29340	Heat-inducible transcriptional repressor HrcA	Normal
	R118-3	REQ_26260	Putative MarR family transcriptional regulator	Reduced
	M79-17	REQ_06490	TetR family transcriptional regulator	Normal
Transport of small molecules	M165-11	REQ_25710	Putative MFS transporter	Normal
Cell wall, LPS, and capsule	M134-23	REQ_45210	Putative D-alanyl-D-alanine carboxypeptidasemetabolism	Normal
Membrane proteins	M74-13	REQ_00210	Putative membrane protein	Normal
	M154-10	REQ_14130	Putative integral membrane protein	Normal
Energy metabolism	M77-23	REQ_32930	Putative nitrite reductase large subunit NirB1	Normal
Non-ribosomal peptide synthesis	M60-10	REQ_23810	Putative non-ribosomal peptide synthetase	Normal
	M70-16	REQ_23810	Putative non-ribosomal peptide synthetase	Normal
	M87-15	REQ_23810	Putative non-ribosomal peptide synthetase	Normal
	M122-3	REQ_23810	Putative non-ribosomal peptide synthetase	Normal
	M162-21	REQ_23810	Putative non-ribosomal peptide synthetase	Normal
	M158-13	REQ_23810	Putative non-ribosomal peptide synthetase	Normal
	M56-20	REQ_35940	Putative non-ribosomal peptide synthetase	Normal
Putative enzyme	M45-4	REQ_00880	Putative short chain dehydrogenase	Normal
	M43-12	REQ_28270	Putative carotenoid oxygenase	Normal
	M124-23	REQ_41020	Short chain dehydrogenase	Normal
	M151-1	REQ_15620	Short chain dehydrogenase	Normal
	M161-16	REQ_10430	Oxidoreductase	Normal
Hypothetical, unclassified, or unknown	M6-19	REQ_26170	Hypothetical protein	Normal
	M82-16	REQ_26170	Hypothetical protein	Normal
	M129-14	REQ_38700	Conserved hypothetical protein	Normal
	M130-19	REQ_38850	Conserved hypothetical protein	Normal
	M153-13*	REQ_27330/REQ_ 27340	Putative HNH endonuclease/putative Fis family transcriptional regulator	Normal
	M162-10*	REQ_12930/REQ_12940	Hypothetical protein/putative secreted esterase	Normal
	M168-10	REQ_23910	Hypothetical protein	Normal
	M182-17	REQ_38870	Hypothetical protein	Normal
Virulence plasmid	M58-1		IcgA	Reduced
	M85-1		IcgA	Reduced
	M96-2		IcgA	Reduced
	M142-10		IcgA	Reduced
	M173-14		IcgA	Reduced
	M175-7		IcgA	Reduced
	M162-10*		IcgA/VapH	Reduced
	M175-8		VirR	Reduced
	M112-17		VirS	No
	M114-17		VirS	No
	M120-9		VirS	No
	M57-20		Orf25	Normal
	M115-3		Putative conjugative transfer TraG/TraD family protein	Normal
	M168-5*		ResA/ParB	Reduced
	M169-21*		VapG/Orf3	Normal

*These mutants had an transposon inserted in the intergenic region between two genes indicaded.