Fig. 2.

FD rat tibial nerve possesses extensive lipid accumulation in axons. (A) Representative TEM images of WT (left) and FD (right) osmophilic accumulation pathology. (B) Representative LM images of normal (top) and lipid accumulated axons (bottom). (C) Manual analysis of accumulation pathology. (D) Representative immunofluorescent images of wild-type (WT) and FD rat proximal tibial nerve. (E) Examples of negative, IB4+ (Gb3), and LAMP1+ axons taken from Fig. 2D. (F) Quantification of IB4+ (Gb3) and (G) LAMP1+ axons in FD compared to controls. Each dot represents the average value of one animal. Light microscopy, n = 6 animals per genotype, immunofluorescence, n = 3 animals per genotype, ** p < 0.01, **** p < 0.0001.