Figure 3.

Enhanced myelopoiesis in mice with apoE2-expressing myeloid cells. Blood was obtained from WT and ApoE^−/− (EKO), APOE2, APOE3, and APOE4 gene replacement mice (N = 8–10 in each group) for analysis by automatic white blood cell counter to determine the number of (A) total white blood cells in WT and KO mice; (B) neutrophils in WT and KO mice; (C) monocytes in WT and KO mice; (D) lymphocytes in WT and KO mice; (E) total white blood cells in APOE2, APOE3, and APOE4 gene replacement mice; (F) neutrophils in the gene replacement mice; (G) monocytes in the gene replacement mice; and (H) lymphocytes in the gene replacement mice. I, bone marrow cells isolated from the gene replacement mice (N = 6 for apoE2 and apoE3 and N = 4 for apoE4 mice) were cultured for 7 days in the presence of cytokines to promote granulocyte/monocyte colony formation to determine GM-CFU. Statistical significance was evaluated by comparing WT and EKO mice with Student's t test. Comparisons between APOE gene replacement mice were evaluated by one-way ANOVA with Student–Newman–Keuls post hoc test. apoE, apolipoprotein E;