Figure 5.

Increased lipid rafts in apoE-deficient and apoE2- and apoE4-expressing macrophages. Peritoneal macrophages isolated from WT, ApoE^−/− (EKO), APOE2, APOE3, and APOE4 gene replacement mice were stained for GM1 with cholera toxin to identify lipid rafts. Representative images (scale bar = 50 μm) of data from four experiments are shown. Statistical significance was evaluated by comparing WT and EKO mice with Student's t test. Differences between APOE gene replacement mice were evaluated by one-way ANOVA with the Student–Newman–Keuls post hoc test. apoE, apolipoprotein E.