FIG. 5.
p68 interacts with the hERα A/B domain but not with the LBD in vivo. p68 interacts with the hERα A/B domain in the mammalian two-hybrid system. A mammalian two-hybrid system with GAL4-p68 fusion protein and VP16-ER fusion proteins (VP16-HE15 and VP16-LBD) was used in COS-1 cells. COS-1 cells were cotransfected with 1 μg of either GAL4-DBD, GAL4-p68, VP16-HE15, or VP16-LBD in the presence or absence of E2 (10−7 M), along with 2 μg of 17M2G-CAT reporter plasmid. Significant interaction was detected only between p68 and the hERα A/B domain.