Table 1.
Primer sequences for real-time qPCR assays.
| Gene | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
| TNF-α | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG |
| IL-1β | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAAC |
| IFN-γ | ATGAACGCTACACACTGCATC | CCATCCTTTTGCCAGTTCCTC |
| GFAP | CCCTGGCTCGTGTGGATTT | GACCGATACCACTCCTCTGTC |
| POMC | GGCTTGCAAACTCGACCTC | TGACCCATGACGTACTTCCG |
| CART | ACCTTTGCTGGGTGCCCGTG | TGCAACGCTTCGATCAGCTCC |
| AgRP | GAGTTCCCAGGTCTAAGTCTGAATG | ATCTAGCACCTCCGCCAAAG |
| NPY | TACTCCGCTCTGCGACACTA | TCTTCAAGCCTTGTTCTGGG |
| DIO2 | AATTATGCCTCGGAGAAGACCG | GGCAGTTGCCTAGTGAAAGGT |
| PPARGC1α | TATGGAGTGACATAGAGTGTGCT | CCACTTCAATCCACCCAGAAAG |
| CIDEA | TGCTCTTCTGTATCGCCCAGT | GCCGTGTTAAGGAATCTGCTG |
| PRDM16 | TGCTGACGGATACAGAGGTGT | CCACGCAGAACTTCTCGCTAC |
| COX7A1 | CAGCGTCATGGTCAGTCTGT | AGAAAACCGTGTGGCAGAGA |
| COX8B | TGTGGGGATCTCAGCCATAGT | AGTGGGCTAAGACCCATCCTG |
| UCP1 | CTGCCAGGACAGTACCCAAG | TCAGCTGTTCAAAGCACACA |
| Lep-R | TGGTCCCAGCAGCTATGGT | ACCCAGAGAAGTTAGCACTGT |
| GAPDH | AGGTCGGTGTGAACGGATTTG | AGTAGACCATGTAGTTGAGGTCA |
TNF-α tumor necrosis factor alpha; IL-1β interleukin 1 beta; IFN-γ interferon gamma; GFAP glial fibrillary acidic protein; POMC proopiomelanocortin; CART cocaine and amphetamine regulated transcript; AgRP agouti-related peptide; NPY neuropeptide Y; DIO2 iodothyronine deiodinase 2; PPARGC1α peroxisome proliferator-activated receptor gamma coactivator 1 alpha; CIDEA cell death-inducing DNA fragmentation factor alpha-like effector A; PRDM16 PR/SET domain 16; COX7A1 cytochrome c oxidase subunit 7A1, COX8B cytochrome c oxidase subunit 8B, UCP1 uncoupling protein 1; Lep-R leptin receptor; GAPDH glyceraldehyde 3-phosphate dehydrogenase.