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. 2021 Sep 13;11:18135. doi: 10.1038/s41598-021-97623-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Volcano plot showing different levels of salivary metabolites in young and elderly subjects. The x-axis is the log2 fold change, whereas the y-axis is the -log10 p value of the Mann Whitney U-test. Two vertical dashed lines show the border of 0.66- and 1.5-fold change, respectively. The horizontal dashed line shows a p value = 0.05. Plots represent significantly higher (red), lower (blue), and unchanged metabolites (gray) in the elderly group. Twenty-one metabolites manifesting age differences are listed in the purple box.