Table 3.

Linear range, determination coefficients, LODs, LOQs and matrix effects of target analytes in plasma

	IS	Linear range (μg/ml)	Linear equation	Linearity (R2)	LOD (μg/ml)	LOQ (μg/ml)	Matrix effect (%) (n=6)
GLYP	GLYP13C215N	0.05–5	y = 0.3388x – 0.0005	0.999	0.02	0.05	109
	AP-4		y = 0.2973x + 0.0045	0.999
	AP-5		y = 0.249x + 0.0029	0.997
GLUF	GLYP13C215N	0.05–5	y = 0.246x + 0.0067	0.998	0.02	0.05	104
	AP-4		y = 0.2157x + 0.0096	0.999
	AP-5		y = 0.1806x + 0.0074	0.999
AMPA	GLYP13C215N	0.05–5	y = 0.1101x + 0.0065	0.997	0.02	0.05	116
	AP-4		y = 0.0965x + 0.0075	0.998
	AP-5		y = 0.0809x + 0.006	0.996
MPPA	GLYP13C215N	0.05–5	y = 0.2801x – 0.0007	0.997	0.02	0.05	140
	AP-4		y = 0.2459x + 0.0033	0.993
	AP-5		y = 0.2061x + 0.002	0.989
GLYP13C215N							106
AP-4							105
AP-5							95.4

LOD: limits of detection

LOQ: limits of quantification