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. 2021 Sep 13;11:18119. doi: 10.1038/s41598-021-97429-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Neighbor-joining analysis of the Gp43 partial DNA sequences revealed several haplotypes. The size of the spheres is proportional to the number of individuals in each circle (numbers are shown inside the spheres). Median vectors (red open rings) represent missing or extinct species. Numbers between haplotypes indicate mutational steps. Large number of mutations were observed between Paracoccidioides lutzii (Plut-green) and P. loboi (Plob-yellow) (n = 54), between these 2 haplotypes and P. americana (Pame-brown) and P. cetii (Pcet-beige) (n = 52). Thirty-four mutations separate P. americana from P. cetii. In contrast, few mutations were observed between these four haplotypes and the remaining Paracoccidioides species (Pbra = P. brasiliensis [blue], Pres = P. restrepiensis [pink], Pven = P. venzuelensis [green]) (n = 11). Three Japanese dolphin haplotypes (Pcet = P. cetii [beige 1 to 3]) showed three missing or extinct haplotypes between them and the four USA dolphins (Pcet = P. cetii 4–7-beige) in this study.