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. 2021 Sep 13;11:18134. doi: 10.1038/s41598-021-97447-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Verification of species identity of all colonies collected as Oculina patagonica, using both genomic (CO1 gene sequencing) and morphological features. The evolutionary history was inferred using the Neighbour-Joining method and evolutionary distances were computed using the Maximum Composite Likelihood method (units: number of base substitutions per site) using the software MEGA X. Images (A–C) are of shallow colonies and (D–F) deep colonies. CS Calyx spacing, CD Calyx diameter, SW Septa width. Scales bar lengths are 1 cm (A, D, in situ), 1 mm (B, E, binocular microscopy), and 100 μM (C, F, scanning electron microscopy, mag. × 60). Graphs in the right panel display measured skeletal features on O. patagonica from 30 m ('Deep', blue boxes) and 2 m ('Shallow', red boxes) water depth. Horizontal black lines within boxes are median values and box limits represent 1st and 3rd quartiles. Whiskers represent 1.5 times the interquartile range. Round black points are individual sample data. No statistically significant differences were present between deep and shallow corals (Student’s t-test, p ≥ 0.05).