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. 2021 Sep 13;11:18131. doi: 10.1038/s41598-021-97443-9

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Tc-β₂t, Tc-rad50 and Tc-eno are expressed specifically in the testes of T. castaneum. RT-PCR-based transcript analysis of cDNA prepared from white-eye mutant T. castaneum adults. T. castaneum RpSL32 housekeeping gene was used to confirm the quality of each cDNA. Lane (M) indicates 50 bp DNA Ladder Catalog No. (N3236S) New England Biolabs.