Figure 2.

Schematic diagram of Tc-β₂t, Tc-rad50 and Tc-eno loci and corresponding transformation constructs. Schematic diagrams of the Tc-β₂t genomic locus and piggyBac-β₂t donor plasmid (A), the Tc-rad50 locus and piggyBac-Rad50 donor plasmid (B), and the Tc-eno locus and piggyBac-Eno donor plasmid (C). All three constructs contained the 3XP3-DsRED cassette as a visual marker and piggyBac inverted repeats (RH and L) for transposase mediated integration in T. castaneum genome. tTA adv and EGFP were separated by the P2A site in all tested plasmids. P, putative promoter region; T, putative terminator region. Dotted lines indicate the upstream and downstream genomic regions used as promoter and terminator respectively to drive the tTA-P2A-EGFP expression in vector construction.