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. 2021 Sep 13;11:18131. doi: 10.1038/s41598-021-97443-9

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transcription of the EGFP reporter in transgenic strains is testes-specific. cDNA from Tc-β₂t-EGFP#2, Tc-Rad50-EGFP#1 and Tc-Eno-EGFP#1 transgenic lines was used to perform the RT-PCR. T. castaneum RpSL32 served as a control for cDNA quality (same samples as shown in Fig. 1). Lane#M is 50 bp DNA Ladder Catalog No. (N3236S) New England Biolabs.