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. 2021 Sep 13;11:18131. doi: 10.1038/s41598-021-97443-9

Table 1.

Summary of piggyBac based transformation experiments.

Exp Donor plasmid No of embryos injected Hatched larvae Number of adult G0 Female/male G0* DsRED+ (#) G1
1 piggyBac2t 1640 152 80 42/38 15
2 piggyBac-Rad50 1750 151 101 58/43 13
3 piggyBac-Eno 1260 113 52 25/37 7

*Crossed the G0 putative transgenic female with w−/− male in single pair cross or in pool of two to four beetles and vice versa. G1 larvae were screened for the DsRED fluorescence in eye.