Fig. 4. Clonal structures of TATs vs primary tumours in all relapsed patients and patients with premalignant lesions in their TATs.

VAF variant allelic frequency; mutations are labelled with different colours according to their clonal type in TATs and primary tumours, indicated at the bottom right. Mutations with VAF ratio >0.8 are defined as clonal, while other mutations are defined as subclonal. Arrows with different colours indicate the clones that are detected in post-operative plasma and saliva: purple, post-operative plasma; blue, post-operative saliva. Patients with premalignant lesion diagnosed pathologically in TATs are labelled with star icons, while relapsed patients are labelled with hexagon icons.