Table 1.
List of primary and secondary antibodies used in this study.
| Antibody | Source | Dilution | |
|---|---|---|---|
| IHC | WB | ||
| Primary antibodies | |||
| Rabbit anti-collagen I | Abcam, Cambridge, UK | 1:500 | |
| Rabbit anti-laminin | Sigma, Poole, UK | 1:400 | |
| Mouse anti-ED1 | Serotec, Kidlington, UK | 1:200 | |
| Mouse anti GFAP | Sigma, Poole, UK | 1:400 | |
| Mouse anti-GAP43 | Invitrogen, Paisley, UK | 1:400 | |
| Mouse anti-NF200 | Sigma, Poole, UK | 1:400 | |
| Rabbit anti-tPA | Abcam, Cambridge, UK | 1:500 | |
| Rabbit anti-PAI-1 | Abcam, Cambridge, UK | 1:500 | |
| Mouse anti-β-actin | Sigma, Poole, UK | 1:1000 | |
| Secondary antibodies | |||
| Alexa488-anti-rabbit IgG | Invitrogen, Paisley, UK | 1:400 | |
| Alexa488 anti-mouse IgG | Invitrogen, Paisley, UK | 1:400 | |
| Alexa594 anti-mouse IgG | Invitrogen, Paisley, UK | 1:400 | |
| Alexa594 anti-rabbit IgG | Invitrogen, Paisley, UK | 1:400 | |
| HRP anti-rabbit IgG | GE Healthcare, Hertfordshire, UK | 1:1000 | |
| HRP anti-mouse IgG | GE Healthcare, Hertfordshire, UK | 1:1000 | |
IHC immunohistochemistry, WB western blot.