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. 2021 Sep 13;11:18173. doi: 10.1038/s41598-021-97352-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Results of ROS level in cell (A), H₂O₂ in cell medium (B) and GSH level (C) in cell lysate. The levels of IL-1β (D), IL-6(E), TNF-α (F) in cell culture medium from RAW264.7 and RAW264.7-IRG1KO with different treatments (24 h). White bar, RAW264.7; black bar, RAW264.7-IRG1KO. LPS, lipopolysaccharide; ITA, itaconic acid; NAC, N-acetyl-L-cysteine. Relative ROS amount in cells with different treatments was normalized to RAW264.7 control and represents the fold change. Values represent the mean ± S.E.M. *, p < 0.05; **, p < 0.01.