Fig. 2. Propagation of colorectal organoids and tumoroids in low-viscosity matrix suspension culture.

a–b Comparison of (a) propagation rates and (b) times (passage 1 to 2) between LVM suspension and dome culture methods for organoids (n = 23 and n = 39, respectively) and tumoroids (n = 29 and n = 25, respectively). c–e Comparison of propagation times for organoids and tumoroids grown in LVM suspension or dome culture according to (c, d) location and (e) tumor stage. f–g Comparison of live-cell yield for (f) organoids and (g) tumoroids from three patients grown for 14 days in LVM suspension or dome culture; scale bars, 200 μm. Data (b, f, g) are plotted as mean ± s.d. Statistical significance (b, f, g) was attributed to values of p < 0.05 as determined by the Student’s t test. NS, p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. N normal, T tumor.