Table 3.

Examples of nanozyme-based biosensors utilizing robust nature of nanozymes capable of possessing catalytic activity at broad range of pH and temperature

Nanozyme/catalyst	Mimicking activity	Target	Type of biosensor	Working condition of nanozyme		Optimal condition for biosensing		References
				pH	Temperature (°C)	pH	Temperature (°C)
Fe3O4	Peroxidase	Blood glucose	Colorimetric	2–11	30–80	7.4	30	[260]
GO-Fe3O4	Peroxidase	Glucose	Colorimetric	3–5	30–50	4	40	[261]
Fe3O4	Peroxidase	Urinary protein	Fluorescence	–	–	7	37	[262]
γ-Fe2O3	Peroxidase	H2O2, Glucose	Colorimetric	2–8	5–50	5	30	[263]
Au@Pt NR-antigen	Peroxidase	Measles virus	Colorimetric (ELISA)	3–9	20–80	3–9	20–80	[264]
Pt–DNA complexes	Peroxidase	H2O2	Colorimetric	–	–	5	25	[251]
VS2 nanosheets	Peroxidase	Glucose	Colorimetric	2–7	20–80	4	RT	[258]
DNA/MoS2 nanosheets	Peroxidase	CEA	Colorimetric	2–5	30–60	4	55	[265]
Single-atom dispersed CNT/FeNC	Peroxidase	Glucose, H2O2	Colorimetric	2.5–5.5	0–60	3.5	50	[54]
CH-Cu	Laccase-like	Phenolic compounds	Colorimetric	3–9	-18–90	6.8	85	[259]
Natural Laccase enzyme	Laccase	Phenolic compounds	Colorimetric	5–8	-18–60	6.8	40	[259]
CoO-OMC	Peroxidase	Glucose, H2O2	Colorimetric	3–5	25–50	4.5	37	[266]
Fe3O4@SiO2@Au MNPs	Peroxidase	Glucose,	Colorimetric	2.5–4	50–75	3	70	[254]
HRP	Peroxidase	H2O2	–	–	–	3.5	40	[50]
Catalase from bovine liver	Catalase	H2O2	Electrocatalytic	–	–	8	45	[92]
Co3O4	Catalase	H2O2	Electrocatalytic	8–10	40–55	> 8–10	> 45	[92]