TABLE 3.
Differences scanners used for multiplex analysis.
| Company | Scanner type | Image acquisition and scanning instrument | Corporate location/notes | Resolution | Image extraction | File type | Automatization |
|---|---|---|---|---|---|---|---|
| Leica Biosystems | BF and FL | Aperio Versa | Illinois, United States | 0.468 μm per pixel with ×20 objective. 0.2um at 40x | WS | TIFF, JPEG | Semi-auto and auto |
| 3DHistech | BF and FL | Pannoramic 250 FLASH III | Budapest, Hungary | 0.172 and 0.087/0.325 and 0.162 pixels | WS | --- | Auto |
| Ventana/Roche | BF and FL | iScan | United States, International | 24-bit true color | WS | TIFF/BIF | Auto and manual |
| PerkinElmer | MSI (BF and FL) | Vectra/Vectra Polaris | Boston, United States | 10× (1.0 μm/pixel), 20× (0.5 μm/pixel) and 40× (0.25 um/pixel) | ROI | QPTIFF, IM3, JPEG, single-layer TIFF, BMP, PNG | Touchless automation with walk-away image acquisition |
| Olympus America | BF and FL | VS110, Nanozoomer (United States) | Japan, International | 0.32 µm/pixel (20×/NA 0.75) - 0.16 µm/pixel (40×/NA 0.95) | WS | Compress images and save images in different file formats | Auto |
| Zeiss | BF and FL | AxioVision MosaiX | United States, Germany | 5× (2.11 µm/pixel), 10× (1.05 µm/pixel), and 20× (0.53 µm/pixel) | WS | AVI, BMP, J2K, JP2, JPG, LSM, MOV. PCT, PCX, PNG, PSD, TGA, TIF, WMF | Auto/manual |
BF: bright field; FL: fluorescence: MALDI: matrix-assisted laser desorption/ionization; FOV: field of view; WS: whole section; ROI: region of interest.