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Composition of solution (mM).
| Composition | Regular solution | Hypertonic-buffered solution |
| Sodium chloride (NaCl) | 140 | 140 |
| HEPES | 10 | 10 |
| D-Glucose | 10 | 10 |
| Potassium chloride (KCl) | 5 | 5 |
| Magnesium chloride (MgCl2) | 1 | 1 |
| Calcium chloride (CaCl2) | 1 | 1 |
| pH | pH 7.4 | pH 7.4 |
| Osmolarity | 300 mOsm/L | 400 mOsm/L |
