TABLE 1.
S. cerevisiae strains used in these experiments
| Strain | Genotype | Reference or source |
|---|---|---|
| W303a | MATa ade2-1 leu2-3,112 ura3-1 his3-11,15 trp1-1 can1-100 ssd1-1 | 54 |
| 169ts | MATα ade2-1 leu2-3,112 ura3-1 his3-11,15 can1-100 ssd1-1 ypt6-1 | 28 |
| JV7-2a | As W303, rpl30Δ::HIS3 (pYE: CEN, URA3 GAL1-RPL30) | 58 |
| BL17 | MATa/MATα YPT6/ypt6-1 rpl30Δ::HIS3/RPL30 his3-11,15/his3-11,15 ura3-1/ura3-1 leu2-3,112/leu2-3,112 ade2-1/ade2-1 can1-100/cam1-100 ssd1-1/ssd1-1 (pRS316: CEN, URA3 ACT1-RPL30) | This study |
| BL174 | ypt6-1 rpl30Δ::HIS3 (pRS316: CEN, URA3 ACT1-RPL30 [construct A]) | This study |
| BL175 | ypt6-1 rpl30Δ::HIS3 (pRS316: CEN, URA3 ACT1-RPL30 [construct B]) | This study |
| BL176 | ypt6-1 rpl30Δ::HIS3 (pRS316: CEN, URA3 ACT1-RPL30 [construct C]) | This study |
| BL177 | ypt6-1 rpl30Δ::HIS3 (pRS316: CEN, URA3 ACT1-RPL30 [construct D]) | This study |
| Y260 | MATa ura3-52 leu2 rpb1-1 | 42 |
| JW1201 | MATα ypt6-1 ade2-1 leu2-3,112 ura3-1 his3-11,15 can1-100 ssd1-1 act1Δ::URA3 RPL30-ACT1 | This study |
| YDS2 | MATa leu2 ade2 his3 trp1 ura3 (∼W303) | D. Shore |
| YDS714 | MATα leu2 ade2 his3 trp1 ura3 sir2Δ::HIS3 | D. Shore |
| JW1210 | MATα leu2 ade2 his3 trp1 ura3 sly1-1 sir2Δ::URA3 | This study |
| JW1211 | MATα leu2 ade2 his3 trp1 ura3 sly1-1 hst1Δ::URA3 | This study |
| YDS430 | MATα leu2 ade2 his3 trp1 ura3 sir3Δ::LEU2 | D. Shore |
| BL180 | YPT6 SIR3 | This study |
| BL181 | YPT6 sir3Δ::LEU2 | This study |
| BL182 | ypt6-1 SIR3 | This study |
| BL183 | ypt6-1 sir3Δ::LEU2 | This study |
| YDV122 | MATα leu2 ade2 his3 trp1 ura3 sir4Δ::LEU2 hmrΔ::TRP1 | D. Shore |
| BL185 | YPT6 SIR4 | This study |
| BL186 | YPT6 sir4Δ::LEU2 | This study |
| BL187 | ypt6-1 SIR4 | This study |
| BL188 | ypt6-1 sir4Δ::LEU2 | This study |