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. 2021 Aug 31;12:726895. doi: 10.3389/fphys.2021.726895

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Copyright © 2021 Scalco, Moro, Mongillo and Zaglia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Three-dimensional imaging of the neuronal network in the murine myocardium. (A) Maximum intensity projection of multiphoton image stacks acquired along 400 μm in tissue clarified LV blocks from the EPI and ENDO regions of a α-MHC/ChR2-td-Tomato mouse heart, stained with an antibody to TH. Red emission of td-Tomato was used to identify CM membrane. (B) Representative single optical section of a sample processed as in (A), resolving the neuro-cardiac interactions, and used for quantification of neuronal processes/cell in EPI and ENDO regions (C). (A–C) Modified with permission from Pianca et al. (2019).