Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 31;12:726895. doi: 10.3389/fphys.2021.726895

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Scalco, Moro, Mongillo and Zaglia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Three-dimensional imaging of the neuronal network in the human myocardium. Topology of the SN network, reconstructed with 3-D rendering of 600 images acquired with a multiphoton microscope along 200 μm, upon whole-mount immunofluorescence in 1 mm³ LV blocks from the EPI region, stained with anti-TH antibody. Images were acquired with an 18× objective, 1.1 NA, allowing a large field of view (850 × 850 μm) at high resolution. A segment of (850 × 850 × 200) μm was imaged. mage series were acquired along the Z-axis, with a step size of 1.5 μm and processed and analyzed with a software for 3-D rendering (Imaris). Images modified with permission from Pianca et al. (2019).