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. 2021 Aug 31;11:661288. doi: 10.3389/fonc.2021.661288

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Copyright © 2021 Gao, Li, Liu, Zhang, Gao and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Role of MIR4435-2HG inhibition on growth, metastasis, and epithelial–mesenchymal transition (EMT) of gastric carcinoma cells. (A) qRT-PCR was applied to test MIR4435-2HG after constructing si-MIR4435-2HG in SGC-7901 and MGC-803 cells. (B) qRT-PCR was applied to test MIR4435-2HG in cells transfected with si-MIR4435-2HG#1 in SGC-7901 and MGC-803 cells. (C) Cell Counting Kit-8 (CCK-8) test was applied to test the proliferation of gastric carcinoma cells transfected with si-MIR4435-2HG#1 in SGC-7901 and MGC-803 cells. (D, E) Transwell test was applied to test the invasion and migration of gastric carcinoma cells transfected with si-MIR4435-2HG#1 in SGC-7901 and MGC-803 cells. (F) Flow cytometry was applied to test the apoptosis of gastric carcinoma cells transfected with si-MIR4435-2HG#1 in SGC-7901 and MGC-803 cells. (G) Western blotting experiment was applied to test the changes of EMT protein in cells. (H) qRT-PCR was applied to test the mRNA changes of EMT markers in cells in SGC-7901 and MGC-803 cells. * indicates p < 0.05; ** indicates p < 0.01.