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. 2021 Aug 31;11:661653. doi: 10.3389/fonc.2021.661653

Figure 2.

Figure 2

Effects of regulating miR-9-5p on biological function of TMZ-resistant glioma cells. (A) qRT-PCR was used to detect the relative expression of miR-9-5p after constructing miR-9-5p-mimics plasmid. (B) qRT-PCR was used to detect the relative expression of miR-9-5p in U87-TMZ/U251-TMZ cells transfected with miR-9-5p-mimics. (C) MTT assay was used to detect the changes of U87-TMZ/U251-TMZ cell activity and IC50 after transfection of miR-9-5p-mimics. (D) Flow cytometry was applied to detect the change of apoptosis rate of U87-TMZ/U251-TMZ cells after transfection of miR-9-5p-mimics. (E) WB test was used to detect the changes of Bcl-2, Bax, cle-caspase3, PARP and cyt-c protein in U87-TMZ/U251-TMZ cells after transfection of miR-9-5p-mimics. * indicates P < 0.05; ** indicates P < 0.01.