Skip to main content
. 2021 Aug 31;12:698045. doi: 10.3389/fimmu.2021.698045

Figure 4.

Figure 4

Inactivated F. nucleatum and E. coli augment secretion of pro-inflammatory cytokines and MMPs by HTR8/SVneo cells. Bar graphs represent secretion of cytokines and matrix metalloproteinases (MMP) by trophoblast cell lines after stimulation with F. nucleatum normalized to respective unstimulated controls (A, B). Data are presented as mean ± SEM. *padj < 0.05; **padj < 0.01; ***padj < 0.001 as analysed by Repeated Measures ANOVA with Dunnett’s multiple comparison post test, comparing each treatment against the corresponding control. Experiments were performed 5 (IL-8, MMP-2 and IL-6 in HTR8/SVneo) or 6 (CXCL1, MMP-9 and IL-6 in BeWo) times in duplicate. Each point represents the mean value of the replicates for each experiment. Ctl: control; Fus: ratio of F. nucleatum to cell number (if no number given ratio is 1); E. coli 1: ratio of E.coli to cell number = 1.