Skip to main content
. 2021 Aug 31;12:698045. doi: 10.3389/fimmu.2021.698045

Figure 5.

Figure 5

BeWo and JEG-3 cells, but not HTR8/SVneo cells express high levels of E-cadherin. IL-6 secretion in response to bacterial stimulation of HTR8/SVneo is partially TLR4 dependent. Bar graphs show E-cadherin expression in trophoblast cell lines normalized to HTR8/SVneo (A). E-cadherin expression was normalized to cell number detected by cell nuclei staining with DRAQ5. Illustrative image of fluorescence signals of DRAQ5 binding and E-cadherin In-Cell Western analysis (B). IL-6 secretion was assessed in HTR8/SVneo after stimulation with F. nucleatum in the presence or absence of a TLR4-blocking antibody (C). The presence of the activated form of IKKα on HTR8/SVneo and BeWo cells was assessed after stimulation with F. nucleatum or LPS (D). Data are presented as mean ± SEM. The experiment was performed once in sextuplicate (A), six times in triplicate (C) or five times in duplicate (D). *padj < 0.05; **padj < 0.01; ns, not significant, as analysed by Repeated Measures ANOVA with Dunnett’s (C) or Šidák’s (D) multiple comparison post test. Data comparison in (C) was performed on F. nucleatum treated cells employing the group without TLR4-blocking antibody as control (“Fus” column).