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. 2021 Aug 31;12:698045. doi: 10.3389/fimmu.2021.698045

Figure 6.

Figure 6

Inactivated F. nucleatum induces NF-κB and β-catenin nuclear translocation. Immunofluorescence of NF-κB (top; green) and β-catenin (bottom; red) of untreated or inactivated F. nucleatum-treated (1 h, MOI = 1) HTR8/SVneo and BeWo cells. Some wells were previously treated with a neutralizing antibody against TLR4 (PAb-hTLR4 (5 µg/mL), the viral inhibitory peptide of TLR4 (VIPER; 5 µM) or Pitstop 2 (known to interfere with E-cadherin/β-catenin signaling) 1 h before bacteria treatment. Nuclei were stained with Hoechst 33258 (blue). Pictures were taken at 60× and the mean fluorescence intensity (MFI) of each channel were quantified in the nuclei (small red circles). All pictures were taken using the same exposure time (green channel: 840 ms; red channel: 400 ms; blue channel: 17 ms). Data (left) depict the MFI (mean ± SEM) of either NF-κB or β-catenin normalized to background (big red circle) for each picture shown. Data comparison was performed by ANOVA Kruskall-Wallis test with Dunns multiple comparison test using F. nucleatum treated cells as control (“Fus” column). *padj < 0.05; **padj < 0.01; ****padj < 0.0001; ns, not significant.