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. 2021 Aug 31;9:733183. doi: 10.3389/fcell.2021.733183

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Copyright © 2021 Lu, Xu, Zeng, Xue, Li, Chen, Zhou, Lin, Liao, Du, Yang and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of HNE, IL-6, TNF-α, and NF-κB p65 in the DCD heart preserved with EVHP. Representative photomicrographs and quantitative analysis of immunohistochemistry for (A) HNE, (B) IL-6, (C) TNF-α, and (D) NF-κB p65 in the DCD heart. Data represent mean ± standard error of the mean. n = 6–8 for each group. DCD, donation after circulatory death; EVHP, ex vivo heart perfusion; HNE, 4-hydroxynonenal; IL-6, interleukin-6; IOD, integrated optical density; TNF-α, tumor necrosis factor-α; NF-κB, nuclear factor kappa-B. ^∗p < 0.05 vs. Control; ^#p < 0.05 vs. DCD-vehicle.