Table 4. Reagents for Cas9:YFP PCR incubation with Taq to add ‘A’ overhangs.

Reagent	Quantity
PCR product (500 ng-5 µg)	500 ng-5 µg
5x reaction buffer*	4 µl
25 mM MgCl2	1.20 µl
10 mM dATPs	0.4 µl
GoTaq polymerase	0.1 µl
Nuclease-free water	Up to 20 µl