Table 6. Reagents for L1 Golden Gate assembly.
| Reagent | Quantity |
|---|---|
| L1 vector | 40 fmol |
| L0 modules/PCR product | 40 fmol of each |
| BsaI | 10 U |
| T4 DNA ligase | 10 U |
| 10x ligation buffer | 2 |
| Nuclease-free water | Make up to 20 µl |
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