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| Reagent | Quantity |
|---|---|
| pICH47732 _TpFCP:NAT | 40 fmol |
| pICH47742_TpFCP:Cas9:YFP | 40 fmol |
| pICH47751_TpU6:sgRNA1 | 40 fmol |
| pICH47761_TpU6:sgRNA2 | 40 fmol |
| pICH41780 (L4E linker) | 40 fmol |
| pAGM4723 (L2 backbone) | 40 fmol |
| BpiI | 10 U |
| T4 DNA ligase | 10 U |
| 10x ligation buffer | 2 µl |
| Nuclease-free water | Up to 20 µl |
