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. 2017 Dec 5;7(23):e2625. doi: 10.21769/BioProtoc.2625

Table 8. Primers for sequencing L2 constructs.

Primer Target Orientation
CCATAAGTCAACGGCTCCAATC FCP promoter Sense
CTCTTGACGACACGGCTTAC NAT Sense
CATTACGGACGAGTACAAGGTG Cas9 Sense
TGAACACGGAGATCACCAAAG Cas9 Sense
CTTCCTGGACAATGAGGAGAAC Cas9 Sense
CAAACTGATCACACAACGGAAG Cas9 Sense
ACTACCTGAGCTACCAGTCC YFP/FCP terminator Sense
GTTTCCGATCTAATGTCCAT sgRNA 2 Anti-sense
TGTGTCGTAATCAAGTATTGC sgRNA 1 Sense