Table 8. Primers for sequencing L2 constructs.
Primer | Target | Orientation |
---|---|---|
CCATAAGTCAACGGCTCCAATC | FCP promoter | Sense |
CTCTTGACGACACGGCTTAC | NAT | Sense |
CATTACGGACGAGTACAAGGTG | Cas9 | Sense |
TGAACACGGAGATCACCAAAG | Cas9 | Sense |
CTTCCTGGACAATGAGGAGAAC | Cas9 | Sense |
CAAACTGATCACACAACGGAAG | Cas9 | Sense |
ACTACCTGAGCTACCAGTCC | YFP/FCP terminator | Sense |
GTTTCCGATCTAATGTCCAT | sgRNA 2 | Anti-sense |
TGTGTCGTAATCAAGTATTGC | sgRNA 1 | Sense |