Figure 9. Guard cell protoplasts in 0.4 M mannitol and 1 mM CaCl2.
Guard cell protoplasts isolated from Arabidopsis. Guard cell protoplasts were resuspended in 200 to 500 µl 0.4 M mannitol and 1 mM CaCl2. The typical yield of guard cell protoplasts was 4.3 x 107 cells per 5,000 leaves with a purity of 98%. The bar represents 10 µm.