Figure 12. Determination of H⁺-ATPase by the immunological method.

Immunodetection of H⁺-ATPase of guard cell after treatment with MG132 ( Yamauchi et al., 2016 ). Guard cell protoplasts from wild-type and aha1-10 mutant were incubated with 50 µM MG132 for 30 min at 24 °C. Upper panel shows immunoblot using H⁺-ATPase antibodies. Lower panel shows Ponceau S staining as loading control. Each lane contains 5 µg guard cell protein.