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. 2021 Aug 31;12:685289. doi: 10.3389/fendo.2021.685289

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Copyright © 2021 Tartakover Matalon, Azar, Meiri, Hadar, Nemirovski, Abu Jabal, Konikoff, Drucker, Tam and Naftali

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FAAH and NAPE-PLD expression in colon biopsies. (A, C) FAAH and (B, D) NAPE-PLD expression were evaluated in colon biopsies using immunohistochemistry. The expression of the proteins in the images was measured using QuPath digital analysis tool. Presented are representative pictures of colon biopsies stained with (A) anti-FAAH and (B) anti-NAPE-PLD. The yellow square in panel (A) illustrates a QuPath reading. The red markings indicate a positive staining for FAAH, while the blue ones indicate staining with hematoxylin. (C, D) Images of FAAH and NAPE-PLD staining from three patients treated with placebo or cannabis at the beginning of the experiment and 8 weeks later.