Figure 1. Xoo preparation.

Spread 50 μl Xoo stock solution (A) to a solid PSA media (B) and incubate at 28 °C for three days until a biofilm is formed (C). Two days before inoculation, transfer Xoo from (B) to a new solid PSA media and incubate at 28 °C for two days (D). At the day of inoculation, make Xoo suspension by diluting Xoo to an OD₆₀₀ of 0.5 from (D) with 10 mM MgCl₂ (E).