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. 2017 Nov 5;7(21):e2603. doi: 10.21769/BioProtoc.2603

Figure 4. Monolayer cultures as well as neurospheres can be differentiated.

Figure 4.

A. Differentiated cells of a monolayer culture with GFAP-positive astrocytes (green) and Map2ab-positive neurons (magenta). B. Differentiated neurosphere culture with β-III-tubulin-positive neurons (magenta) and GFAP-positive astrocytes (green). C. Differentiated neurosphere culture with O4-positive Oligodendrocytes (red). Scale bars = 50 µm.