Table 2. Cell culture volumes and densities.

	Surface area (mm)	Seeding density	Cells at confluency	Accutase	Growth medium	Coating
Dishes
35 mm	962	0.1 x 106	1.2 x 106	0.3 ml	2.0 ml	2.5 ml
60 mm	2.827	0.25 x 106	3.2 x 106	0.5 ml	4.0 ml	3.5 ml
100 mm	7.854	1.0 x 106	8.8 x 106	1.0 ml	10.0 ml	8.0 ml
150 mm	17.671	2.00 x 106	20.0 x 106	2.0 ml	20.0 ml	10.0 ml
Cluster plates
6-well	962	0.9 x 105	1.2 x 106	0.3 ml	2.0 ml	1.2 ml
12-well	401	0.05 x 106	0.5 x 106	0.2 ml	1.0 ml	0.5 ml
24-well	200	0.025 x 106	0.25 x 106	0.1 ml	0.5 ml	0.4 ml
Flasks
T25	2,500	0.25 x 106	3.2 x 106	0.5 ml	5.0 ml	3.0 ml
T75	7,500	1.0 x 106	12.0 x 106	1.0 ml	14. 0 ml	7.0 ml
T160	16,000	3.0 x 106	20.0 x 106	2.5 ml	ml	10 ml