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. 2017 Nov 20;7(22):e2605. doi: 10.21769/BioProtoc.2605

Figure 7. The major cell types of the brain are present and easily detectable by immunofluorescence.

Figure 7.

A. The characteristic Purkinje cells (PC) of the cerebellum (GABAergic neurons), have been detected in mouse cerebellum slice with the Calbindin-28k marker at DIV0. The arrow head shows the soma of the PC; the arrow shows the dendritic tree of the PC and Ax designate the regrouping PC axons. B. The mouse hippocampal neurons have been stained at DIV0 using the specific neuron nuclear marker NeuN to reveal the internal organization of the hippocampus. C. The mouse hippocampal astrocytes have been stained using the specific Glial Fibrillary Astrocyte Protein (GFAP) marker at DIV0. D. The microglia have been stained at DIV7 using the specific marker Iba1 in mouse hippocampus. E. The oligodendrocytes have been stained at DIV5 using the Olig2 specific nuclear marker in mouse hippocampus. CA1: Cornu Ammonis area 1, CA3: Cornu Ammonis area 3, DG: Dentate Gyrus.