Figure 8. Study of morphological rearrangements and kinetic of mouse or hamster OBC viability over 7 days of culture.

A. Hippocampus slices from mice or hamsters were stained with anti-NeuN antibody (neurons marker) at day 0 and day 7 of culture. Pictures show a conserved general organization of the hippocampus over at least 7 days, including the Ammon’s horn (Cornu Ammonis CA) regions and dentate gyrus (DG), which showed only limited loss of cell density (white arrows) possibly related to neuron death. B. Cerebellum slices from mice or hamsters were stained with Calbindin 28K (CB 28K–Purkinje Cells marker) at day 0 and day 7 of culture. Arrows in the pictures show neuronal loss and arrow heads show an interruption of the Purkinje cells layer. Nuclei were counterstained with DAPI. Images taken at the indicated times of culture with a Leica SP5 confocal microscope show morphological evolution within slices at low (reconstructed tile–on left column for each day) or high magnification on right column for each day (20x objective in left panel of each days and 40x objective in right panel of each days corresponding to the white square) over the culture duration.