Figure 3.

The frequency of DC costimulatory molecules CD83, CD86, CD40, and CD80 (A–D) in whole PBL was assessed in normal, BDN, and BD mice. Isolated PBL were surface stained and evaluated by flow cytometry. A representative histogram of CD83+ cells is shown in (E). For statistical analysis, the Kruskal-Wallis test was performed using GraphPad. The number of mice used in the experimental groups was five in the normal, six in the BDN, and five in the BD group. The experiments were performed independently at least three times.