Table 1.
Characteristics and results of the MALDIxin test on P. aeruginosa strains.
| Name of strain | MIC to colistin (mg/L) | Colistin resistance mechanism | PRR MALDIxin results | References |
|---|---|---|---|---|
| Reference strains | ||||
| PAO1 | 1 | – | 0.00±0.00/0.02±0.01* | Tenover, 2000 |
| AB8.2 | 128 | PmrB (V28G) | 0.87±0.25 | Bolard et al., 2019 |
| AB16.2 | 128 | PmrB (Δ172) | 1.17±0.22/2.12±0.07* | Bolard et al., 2019 |
| AB8.4 | 4 | PhoQ (Δ240–247) | 0.00±0.00/0.44±0.02* | This study |
| PAOW2 | 4 | ParR (M59I) | 0.29±0.05/0.28±0.01* | Muller et al., 2011 |
| Colistin susceptible clinical strains | ||||
| 185715 | 1 | – | 0.00±0.00/0.00±0.00* | This study |
| 185716 | 1 | – | 0.00±0.00/0.00±0.00* | This study |
| 218401 | 1 | – | 0.00±0.00/0.00±0.00* | This study |
| 218418 | 1 | – | 0.00±0.00/0.00±0.00* | This study |
| 218419 | 0.5 | – | 0.00±0.00/0.00±0.00* | This study |
| 218420 | 1 | – | 0.00±0.00/0.00±0.00* | This study |
| 218422 | 0.5 | – | 0.00±0.00/0.00±0.00* | This study |
| 218423 | 0.5 | – | 0.00±0.00/0.00±0.00* | This study |
| 218424 | 2 | – | 0.00±0.00/0.07±0.01* | This study |
| 218427 | 1 | – | 0.00±0.00/0.00±0.00* | This study |
| 218428 | 0.5 | – | 0.00±0.00/0.00±0.00* | This study |
| 218429 | 0.5 | – | 0.00±0.00/0.00±0.00* | This study |
| 218435 | 0.5 | – | 0.00±0.00/0.00±0.00* | This study |
| 218437 | 1 | – | 0.00±0.00/0.03±0.01* | This study |
| Colistin resistant clinical strains | ||||
| 142243 | 128 | PmrB, (Q105P)b | 0.00±0.00/0.22±0.01* | Bolard et al., 2019 |
| 152739 | 16 | PmrB (V264A)a | 0.39±0.04 | Bolard et al., 2019 |
| 153038 | 64 | PmrB (D47N)b | 0.67±0.03 | Bolard et al., 2019 |
| 153091 | 128 | PmrA (L21I) | 0.86±0.07 | Bolard et al., 2019 |
| 163795 | 128 | PmrB (G188D)b | 0.23±0.01 | Bolard et al., 2019 |
| 174536 | 4 | PmrB (V136E)b | 0.00±0.01/2.03±0.13* | Bolard et al., 2019 |
| 174660 | 64 | PmrB (G121P, V313A)b | 0.60±0.44 | Bolard et al., 2019 |
| 174782 | 4 | PmrB (H33Y)a | 0.98±0.14 | Bolard et al., 2019 |
| 175058 | 4 | PmrB (D45N, G362S)a | 0.24±0.01 | Bolard et al., 2019 |
| 175101 | 32 | PmrB (R92H, G123S)b | 0.19±0.07 | Bolard et al., 2019 |
| 185345 | 4 | – | 0.00±0.00/0.87±0.07* | This study |
| 185374 | 4 | – | 0.00±0.00/0.22±0.01* | This study |
| 185819 | 128 | PhoQ (R275X) | 0.22±0.06 | This study |
| 196337 | 4 | – | 0.00±0.00/0.34±0.03* | This study |
a
Mutation Y345H associated with polymorphism in the protein PmrB.
b
Mutations S2P, A4T, V6A, V15I, G68S, and Y345H associated with polymorphism in the protein PmrB.
–, no mutation has been identified in genes cprS, cprR, parS, parR, pmrA, pmrB, phoP, phoQ, colS, and colR.
*
PRR obtained after colistin induction of the strains.