FIGURE 6.

Administration of MSC reduces inflammation in hypoxia-induced PH. (A) Immunofluorescence images of DAPI (blue) and CD68 (green) expression in hypoxia-induced mice treated with MSCs. White arrow: CD68⁺ macrophage. (B), Quantification of CD68⁺ macrophages in the lungs of mice. A total of 10 fields of view per mouse were counted. ^∗∗∗P < 0.001. NC, Vehicle control; Hx, hypoxia-induced group; Hx + MSC, MSC-treated group. n = 4–5; Scale bars = 50 μm. (C–F) The mRNA level of iNOS, IL-1β, IL-6, and CXCL10 in lung tissues from NC, Hx, MSC groups’ mice 21 days after corresponding treatment were detected by qRT-PCR. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001. NC, Vehicle control; Hx, hypoxia-induced group; Hx + MSC, MSC-treated group. n = 3–7.