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. 1999 Aug;19(8):5441–5452. doi: 10.1128/mcb.19.8.5441

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Copyright © 1999, American Society for Microbiology

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FIG. 6 — (A) Epitope-tagged Imp3 and Imp4 proteins complement their respective null alleles. Either a triple-MYC or a triple-HA tag was placed at the 3′ end of an IMP3 or an IMP4 gene and cloned into the expression plasmid p415GPD. Strains were constructed so that the only expressed Imp3 or Imp4 protein is that with the indicated epitope tag. Growth on YPD of the epitope-tagged Imp protein strains was compared to that of a similarly constructed strain that expresses Mpp10p from p415GPD (24). (B) Western blotting with anti-HA epitope antibodies. Total protein was isolated from strains expressing HA-tagged Imp proteins and analyzed by SDS-PAGE and Western blotting. As a control, protein was isolated from strains expressing MYC-tagged protein. (C) Western blotting with anti-MYC epitope antibodies. Total protein was isolated from strains expressing MYC-tagged Imp proteins and analyzed by SDS-PAGE and Western blotting. As a control, protein was isolated from strains expressing HA-tagged protein.