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Depletion of the Imp proteins does not affect U3 snoRNA levels. RNA was isolated from pGAL1::IMP3 and pGAL1::IMP4 at the switch from galactose to glucose (time zero) and 24 h after the switch to glucose. As a control, RNA was also isolated from an otherwise isogenic strain, YPH259. Equal amounts of RNA were run on a denaturing polyacrylamide gel, blotted, and hybridized with probes complementary to the U3, snR10, snR190, and U14 snoRNAs.
