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. 2021 Aug 31;22(5):1235. doi: 10.3892/etm.2021.10669

Figure 1.

Figure 1

Successful establishment of an acute glaucoma rat model. (A) Observation of optic neuropathy by haematoxylin-eosin staining after modelling. Magnification, x40. (B) Measurement of class III β-tubulin levels by immunofluorescence staining after modelling. Magnification, x40 (C) Measurement of the levels of cytokines, including IL-4, IL-6, TNF-α, IL-1β, IL-13 and IFN-γ, by flow cytometry after modelling. (D) Assessment of T lymphocyte proliferation by the CFSE method on days 1, 3, 14, and 28 after modelling. The cells were treated with CFSE and analysed by flow cytometry. (E) The expression levels of Th1 and Th2 cytokines determined by flow cytometry on days 1, 3, 7, 14, and 28 after modelling. *P<0.05; **P<0.01; ***P<0.001. IL, interleukin; TNF, tumour necrosis factor; IFN, interferon; CFSE, carboxyfluorescein diacetate succinimidyl ester.